biospin p30 column (Bio-Rad)
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biospin p30 column
Biospin P30 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p30+columns/Bio-Spin+P-30+Gel+Columns/pm41887061-181-10-13
Average 95 stars, based on 150 article reviews
Biospin P30 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p30+columns/Bio-Spin+P-30+Gel+Columns/pm41887061-181-10-13
Average 95 stars, based on 150 article reviews
biospin p30 column - by Bioz Stars,
2026-09
95/100 stars
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Extraction:Article Title: Alternative start codon selection shapes mitochondrial function and rare human diseases Article Snippet: For transcription, 1 μg of purified PCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. .. To remove free nucleotides and abortive transcripts, the reaction was passed through Article Title: Alternative start codon selection shapes mitochondrial function and rare human diseases. Article Snippet: For transcription, 1 μg of purified PCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. .. To remove free nucleotides and abortive transcripts, the reaction was passed through Article Title: Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology Article Snippet: 1 μg of PCR product was used in an in vitro transcription reaction using the HiScribe T7 High Yield RNA Synthesis kit (NEB, E2040S) in the presence of 20units/mL SUPERaseIn. .. Free nucleotides and abortive transcripts were removed using Article Title: Alternative start codon selection shapes mitochondrial function during evolution, homeostasis, and disease Article Snippet: For transcription, 1 μg of purified PCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. .. To remove free nucleotides and abortive transcripts, the reaction was passed through Purification:Article Title: Alternative start codon selection shapes mitochondrial function and rare human diseases Article Snippet: For transcription, 1 μg of purified PCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. .. To remove free nucleotides and abortive transcripts, the reaction was passed through Article Title: Alternative start codon selection shapes mitochondrial function and rare human diseases. Article Snippet: For transcription, 1 μg of purified PCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. .. To remove free nucleotides and abortive transcripts, the reaction was passed through Article Title: Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology Article Snippet: 1 μg of PCR product was used in an in vitro transcription reaction using the HiScribe T7 High Yield RNA Synthesis kit (NEB, E2040S) in the presence of 20units/mL SUPERaseIn. .. Free nucleotides and abortive transcripts were removed using Article Title: Alternative start codon selection shapes mitochondrial function during evolution, homeostasis, and disease Article Snippet: For transcription, 1 μg of purified PCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. .. To remove free nucleotides and abortive transcripts, the reaction was passed through Concentration Assay:Article Title: CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning Article Snippet: .. The permeabilized cells were resuspended in 100 μl of DEPC treated storage buffer (10 mM Tris-HCl pH 8.0, 25% (v/v) glycerol, 5 mM MgCl2, 0.1 mM EDTA and 5 mM DTT) followed by nuclear run-on with 100 μl 2X run-on mix (5 mM Tris-HCl pH 8.0, 2.5 mM MgCl2, 0.5 mM DTT, 150mM KCl, 0.5 mM ATP, 0.5 mM CTP, 0.5 mM GTP, 0.5 mM Br-UTP, RNase inhibitor, 1% Sarkosyl) at 37 °C for 5 min. Total RNA was extracted by Trizol and followed by hydrolyzation with NaOH at a final concentration of 0.2 N on ice for 18 min. After quenching with ice-cold Tris-HCl pH 6.8 at a final concentration of 0.55 M and exchanging buffer via Article Title: Physiological role of the 3’IgH CBEs super-anchor in antibody class switching Article Snippet: .. Hydrolysation was performed by adding NaOH at a final concentration of 0.2 N on ice for 18 min, and followed by quenching with ice-cold Tris-HCl pH 6.8 and exchanging Incubation:Article Title: CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning Article Snippet: .. The permeabilized cells were resuspended in 100 μl of DEPC treated storage buffer (10 mM Tris-HCl pH 8.0, 25% (v/v) glycerol, 5 mM MgCl2, 0.1 mM EDTA and 5 mM DTT) followed by nuclear run-on with 100 μl 2X run-on mix (5 mM Tris-HCl pH 8.0, 2.5 mM MgCl2, 0.5 mM DTT, 150mM KCl, 0.5 mM ATP, 0.5 mM CTP, 0.5 mM GTP, 0.5 mM Br-UTP, RNase inhibitor, 1% Sarkosyl) at 37 °C for 5 min. Total RNA was extracted by Trizol and followed by hydrolyzation with NaOH at a final concentration of 0.2 N on ice for 18 min. After quenching with ice-cold Tris-HCl pH 6.8 at a final concentration of 0.55 M and exchanging buffer via Article Title: Method for identification of the most abundant oligonucleotide species in a library of oligonucleotides Article Snippet: .. Next, the samples were desalted by gel-filtration on Labeling:Article Title: CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning Article Snippet: .. The permeabilized cells were resuspended in 100 μl of DEPC treated storage buffer (10 mM Tris-HCl pH 8.0, 25% (v/v) glycerol, 5 mM MgCl2, 0.1 mM EDTA and 5 mM DTT) followed by nuclear run-on with 100 μl 2X run-on mix (5 mM Tris-HCl pH 8.0, 2.5 mM MgCl2, 0.5 mM DTT, 150mM KCl, 0.5 mM ATP, 0.5 mM CTP, 0.5 mM GTP, 0.5 mM Br-UTP, RNase inhibitor, 1% Sarkosyl) at 37 °C for 5 min. Total RNA was extracted by Trizol and followed by hydrolyzation with NaOH at a final concentration of 0.2 N on ice for 18 min. After quenching with ice-cold Tris-HCl pH 6.8 at a final concentration of 0.55 M and exchanging buffer via Polymerase Chain Reaction:Article Title: Method for identification of the most abundant oligonucleotide species in a library of oligonucleotides Article Snippet: .. Next, the samples were desalted by gel-filtration on Buffer Exchange:Article Title: The Fundamental Role of Chromatin Loop Extrusion in Physiological V(D)J Recombination Article Snippet: .. Extracted RNA was hydrolyzed with NaOH (Final 0.2 N) on ice for 12 min, quenched by ice-cold Tris-HCl, PH 6.8 (Final 0.55 M), followed by buffer exchange with |